Rna Library Prep Illumina

Illumina stranded total rna prep ligation with ribo zero plus illumina stranded mrna prep ligation.

Rna library prep illumina. The ultra ii rna library prep kit for illumina is designed for non directional non strand specific rna library construction and delivers significantly increased sensitivity and specificity from your rna seq experiments from ever decreasing amounts of input rna. The products previously known as truseq stranded total rna library prep kit with ribo zero human mouse rat cat. A condensed version of the illumina stranded total rna prep ligation with ribo zero plus protocol for experienced users. Rapid library preparation from a broad range of sample types for studying the coding and non coding transcriptome with unparalleled study flexibility.

Illumina stranded total rna prep with ribo zero plus. Jul 15 2020. This information is provided for use with illumina instruments only. Oligonucleotide oligo sequences of illumina adapters used in library prep kits.

Truseq rna exome previously known as the truseq rna access library prep kit converts total rna into template molecules of known strand origin followed by sequence specific capture of coding rna. The total yield was 0 91 gb with 97 of bases at or above q30. For the complete rna enrichment prep workflow you ll need to order library prep a probe panel and index adapters separately. 20040556 illumina rna prep with enrichment l tagmentation.

The novel workflow has been optimized to minimize adaptor dimers while producing high yield high diversity libraries. Illumina rna prep with enrichment includes library preparation reagents required to pre capture rna includes reagents for cdna conversion and enrichment probes not included. These libraries were sequenced on the miniseq system using the miniseq high output reagent kit at a 1 x 36 bp read length configuration. Please note that adaptors primers rrna depletion reagents and poly a mrna isolation reagents are not included in the kit and are available separately.

Download 1 mb. Rs 122 2201 rs 122 2202 and rs 122 2203 and truseq stranded total rna library prep kit with ribo zero gold cat. The nebnext ultra rna library prep kit for illumina contains reagents for preparation of non directional rna libraries for illumina sequencing. Download 1 mb.

The human brain reference rna hbrr and universal human reference rna uhrr samples were prepared using the truseq small rna library prep kit. This provides a low cost solution for analyzing human rna isolated from formalin fixed paraffin embedded ffpe tissues and other low quality samples. The nebnext multiplex small rna library prep kit for illumina index primers 1 48 contains the adaptors primers enzymes and buffers required to convert small rnas into indexed libraries for next generation sequencing on the illumina platform.